Majorly, two factors are responsible for the difficulties in the plant DNA extraction: Also, each cell serves a different function in different tissue. Some of the organelles such as mitochondria, endoplasmic reticulum, nucleus and Golgi body etc are similar in all types of tissue but the structure and the composition are different in different cells. The backbone of the DNA is made up a phosphate while the hydrogen bond joins two opposite strands. The DNA is identical in all living organisms, made up of Adenine, Thymine, Cytosine and Guanine. Modification in CTAB DNA extraction protocol.CTAB DNA extraction buffer for plant DNA extraction.Role of liquid nitrogen in plant DNA extraction:.How to extract DNA from the plant cell?.